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Toe-clipping (removal of the first bone of certain toes, corresponding to a predetermined numbering code), as a method of identification of small rodents, should be used only when no other individual identification method is feasible and should be performed only on neonatal mice up to 7 days of age, consistent with the “Guide for the Care and Use of Laboratory Animals” (8th edition, 2011).
Toe clipping can be considered under the following conditions:
IACUC approved: July 2011